Effect of Suanzaoren Tang on Learning-memory Function and NLRP3 Pathway in Sleep-deprived Rats / 中国实验方剂学杂志

Objective:To explore the mechanism of Suanzaoren Tang in improving learning-memory of sleep-deprived rats based on Nod-like receptor 3 （NLRP3） inflammatome pathway. Method:The rats were randomly divided into normal control group， model group， Eszolam group（5.4×10^-4 g·kg^-1·d^-1）， low-dose Suanzaoren Tang group（4.59 g·kg^-1·d^-1）and high-dose Suanzaoren Tang group （18.36 g·kg^-1·d^-1）. In addition to normal control group， other groups were used to constructed sleep-deprived model， which was concurrent with 30-day continuous drug administration. Water maze was used to evaluate the learning-memory function of rats； The mRNA and protein expressions of NLRP3， apoptosis-related speckle proteins （ASC）， aspartic acid-specific cysteine protease-1 （Caspase-1）， interleukin-1（IL-1） and IL-18 in the hippocampus of rats were detected by Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） and Western blot. Result:Compared with control group， the incubation period of the platform， the total distance of swimming and the duration of first reaching the platform in model group were significantly increased （<italic>P</italic><0.01）， while the number of platform crossings and the target quadrant time were decreased （<italic>P</italic><0.01）. Compared with the model group， the incubation period， total swimming distance and the duration of first reaching the platform in low-dose Suanzaoren Tang group and high-dose Suanzaoren Tang group were decreased to different degrees （<italic>P</italic><0.05，<italic>P</italic><0.01）， while the number of platform crossings and the target quadrant time were increased significantly （<italic>P</italic><0.05，<italic>P</italic><0.01），but with no significant change in estazolam group. Compared with normal control group， mRNA and protein expressions of NLRP3， ASC， Caspase-1， IL-1<italic>β</italic>， IL-18 in the hippocampus of the model group were significantly increased （<italic>P</italic><0.05，<italic>P</italic><0.01）. Compared with model group， mRNA and protein expressions of NLRP3， ASC， Caspase-1， IL-1<italic>β</italic> and IL-18 in the hippocampus of the rats in low-dose Suanzaoren Tang group and high-dose Suanzaoren Tang group were all decreased to different degrees （<italic>P</italic><0.05）. The mRNA and protein expressions of NLRP3， ASC， Caspase-1， IL-1<italic>β</italic> and IL-18 in the hippocampus of Suanzaoren group also decreased， but with no significant change. Conclusion:Suanzaoren Tang can improve the learning-memory function of sleep-deprived rats， and its mechanism is related to the inhibition of NLRP3 inflammatome pathway in hippocampus and the alleviation of neuroinflammation.

Effects of Extractive from Gastrodiae Rhizoma on Learning-Memory Function in Mice / 中国中医药信息杂志

Objective To evaluate the effects of extractive from Gastrodiae Rhizoma on acquisition, consolidation and retrieval of learning-memory function in mice;To provide some reference for clinical research and development of new drugs.Methods Male Kunming mice were randomly divided into control group, model group, positive control group and Gastrodia extractive group. Positive control group and Gastrodia extractive group were given gavage by using relevant medicine 0.2 mL/10 g, and the control group and model group were given gavage with the same amount of distilled water for 16 days. After receiving gavage for continuous 11 days, memory acquisition barrier model was induced by scopolamine;memory consolidation barrier model was induced by chloromycetin;memory retrieval barrier model was induced by EtOH. The learning-memory function was reviewed by escape latency and spatial search distance. The quadrant and distance search time percentage was detected through directional navigation test and spatial probe test in Morris water maze.Results Extractive from Gastrodia Rhizoma shortened the time for acquisition, consolidation and retrieval of learning memory about escape latency and spatial search distance (P<0.05,P<0.01), and the quadrant and distance search time percentage were prolonged (P<0.01).Conclusion Extractive from Gastrodia Rhizoa can effectively improve the acquisition, consolidation and retrieval of learning-memory function in mice.